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Genomic Data Processing: Long Read Processing: Beginner

How To Use This Page

This page provides a workflow/pipeline on genomic data processing when only long reads are being used for analysis. The pipeline is described as being beginner level, as it is not differentiating based on the sequencing company and is meant to cover all long read types.

 

To effectively utilize this page, start by exploring the major steps outlined here, which include de novo assembly and alignment/mapping. These steps are thoughtfully presented to assist beginners and new learners in getting started with genomic data analysis. To further streamline your experience, we've divided the workflow based on read coverage and quality. Choose the section that most closely aligns with your data, and follow the subsequent steps as a guide. For each step, we've provided an example of a program that could be employed to accomplish the task. Clicking on the associated link will direct you to the software's manual, offering detailed instructions for its usage.

 

It's important to keep in mind that while this guide serves as a valuable starting point, it is not an exhaustive resource and may need adaptation based on the specific characteristics of your data and other parameters. Genomic research is continually evolving, so remain flexible and open to adjustments as your project progresses.

Assembly / Mapping
De Novo Assembly
Reference Mapping
De Novo Assembly
Reference Mapping
Alignment
Assembly
Assembly
Quality Check
Quality Check
Quality Check
Quality Check
Alignment
Preprocessing
Assessment
Error Correction
Assessment
Trimming
Coverage Depth / Quality
High
≥ 50-100X, Good Quality
Low
< 50X, Poor Quality
Raw Data

Long Reads

Genomic Long Read Processing
Postprocessing
Scaffolding
Quality Check
Quality Check
Polishing
Polishing
Gap Filling
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