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Genomic Data Processing: Hybrid Read Processing

How To Use This Page

This page provides a workflow/pipeline on genomic data processing when both short and long reads are being used for analysis. This is sometimes referred to as a hybrid approach. The pipeline is divided based on whether the data available for processing contains primarily short reads supplemented with long reads, or primarily long reads supplemented with short reads, for increased accuracy. 

 

To effectively utilize this page, start by exploring the major steps outlined here, which include de novo assembly and alignment/mapping. These steps are thoughtfully presented to assist beginners and new learners in getting started with genomic data analysis. For each step, we've provided an example of a program that could be employed to accomplish the task. Clicking on the associated link will direct you to the software's manual, offering detailed instructions for its usage.

 

It's important to keep in mind that while this guide serves as a valuable starting point, it is not an exhaustive resource and may need adaptation based on the specific characteristics of your data and other parameters. Genomic research is continually evolving, so remain flexible and open to adjustments as your project progresses.

Assembly / Mapping
Alignment
Assembly
Assembly
Quality Check
Quality Check
Quality Check
Quality Check
Alignment
Preprocessing
Assessment
Assessment
Raw Data

Short Reads

supplemented with long reads

Long Reads

supplemented with short reads

De Novo Assembly
Reference Mapping
De Novo Assembly
Reference Mapping
Error Correction
Error Correction
Filtering
Error Correction
Error Correction
Filtering
Genomic Hybrid Read Processing
Postprocessing
Scaffolding/Gaps
Quality Check
Quality Check
Polishing
Polishing
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