Skip to Main Content

Genomic Data Processing: Short Read Processing

How To Use This Page

This page provides a workflow/pipeline on genomic data processing when only short reads are being used for analysis. 

 

To effectively utilize this page, start by exploring the major steps outlined here, which include de novo assembly and alignment/mapping. These steps are thoughtfully presented to assist beginners and new learners in getting started with genomic data analysis. For each step, we've provided an example of a program that could be employed to accomplish the task. Clicking on the associated link will direct you to the software's manual, offering detailed instructions for its usage.

 

It's important to keep in mind that while this guide serves as a valuable starting point, it is not an exhaustive resource and may need adaptation based on the specific characteristics of your data and other parameters. Genomic research is continually evolving, so remain flexible and open to adjustments as your project progresses.

Short Read Processing Pipeline

Assembly / Mapping
De Novo Assembly
Reference Mapping
Alignment
Assembly
Quality Check
Quality Check
Choose
Appropriate 
Parameters
Choose
Appropriate 
Parameters
Preprocessing
Assessment

Trimming
Raw Data

Short Reads

Genomic Short Read Processing
Postprocessing
Quality Check
Scaffolding
Sufficient Assembly? 
No
Yes
Complete
Draft
Assembly
Sufficient Mapping? 
Yes
No
Complete
Draft
Assembly
University of Florida Home Page

This page uses Google Analytics - (Google Privacy Policy)

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.